Genetic variation of Indonesian dipterocarps assessed by amplified fragment length polymorphisms

Siregar, Iskandar Z. and Siregar, U.J. and Cao, C.P. and Gailing, O. and Finkeldey, R. (2005) Genetic variation of Indonesian dipterocarps assessed by amplified fragment length polymorphisms. [Conference or Proceeding]

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Abstract

The dipterocarp forests of Indonesia are centres of global biodiversity. The design of efficient conservation strategies requires an understanding of this diversity and its dynamics in time and space at all levels. Variation within species, i.e. genetic variation, is largely unknown for Indonesian dipterocarps, and even patterns of genetic differentiation among species are poorly described. The amplified fragment length polymorphism (AFLP) technique was used to observe genome-wide genetic differentiation among Indonesian dipterocarps and to study levels of genetic diversity and differentiation among populations of two closely related, widely distributed species: Shorea leprosula and S. parvifolia. The results generally support the taxonomic classification within the subfamily Dipterocarpoideae based on morphological traits and previous molecular studies of chloroplast DNA (cpDNA). Considerable genetic variation was observed within the population for both species (mean He = 0.161 for S. leprosula and 0.138 for S. parvifolia). Ranking of locations with regard to variation levels revealed a similar trend; the populations in a single location on Sumatra (“Asialog”) had the highest variation. Genetic differentiation among populations was high (GST = 25% for S. leprosula and 31% for S. parvifolia). The results will help to efficiently select and design genetic resources of Indonesian dipterocarps and contribute to the sustainable utilization of genetic resources. In combination with ongoing studies on the extraction of DNA from dipterocarp wood they will be useful to develop reliable molecular tools to identify the origin of dipterocarp timber and processed wood. These tools are expected to gain importance for the international trade of wood and wood products from dipterocarps and for the observation of the chain-of-custody (COC) within the context of forest certification.

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