Effect of honey supplementation into Tris Extender on cryopreservation of bull spermatozoa

N., Yimer and N, Muhammad and K., Sarsaifi and Y., Rosnina and H., Wahid and A. M., Khumran and A., Kaka (2015) Effect of honey supplementation into Tris Extender on cryopreservation of bull spermatozoa. [Journal / Magazine]

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Abstract

The aim of this study was to evaluate the effects of using honey as a supplement into Tris extender in comparison with a commercial Bioxcell® extender on bull semen cryopreservation quality. Semen samples from four fertile bulls, which comprised of two Friesian Sahiwal, one Braford and one Kedah-Kelantan breeds, were collected using an electro-ejaculation method. Collected samples were freshly evaluated physically and microscopically before divided and diluted using four Tris-based extenders containing 0% (control), 2.5%, 5% and 10% honey, and a commercial Bioxcell® extender. The processed semen was equilibrated at 5°C for 3 h (chilling), packed into straws (0.25 ml), frozen and stored in a liquid nitrogen tank for 24 h before thawing and assessment of quality. Semen quality parameters used for evaluation and comparison after chilling and thawing included general and progressive motility (analyzed using a computer assisted semen analyzer), livability and sperm morphology (done using eosin-nigrosin staining technique). The experiment revealed that among all Tris-based extenders, improved post-cryopreservation semen quality was obtained from Tris+2.5% honey; comparable to Bioxcell® which was superior to all other extenders. Use of honey at concentrations of 5 and 10% however, were not suitable and showed significantly poor semen quality following cryopreservation. Therefore, Tris extender with 2.5% honey could be considered as a cheaper alternative to Bioxcell® as it could be prepared in the laboratory whenever needed.

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