The effect of medium compositions and light on the production of ascorbic acid, α-tocopherol and carotenoids in Centella asiatica callus

Yusuf, Norhayati and Kusnan, Misri and Mohd Fadzillah, Nor’Aini and Mahmood, Maziah (2016) The effect of medium compositions and light on the production of ascorbic acid, α-tocopherol and carotenoids in Centella asiatica callus. Journal of Tropical Plant Physiology, 8. pp. 12-22.

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Abstract

The effect of medium strength, different sucrose and casein hydrolysate (CH) concentrations with varying pH of medium as well as photoperiods and light intensities on the synthesis of ascorbic acid, α-tocopherol and carotenoid content in Centella asiatica callus were evaluated. Full strength Murashige and Skoog (MS) medium with 30 to 50 g L-1 sucrose, with 0.5 to 1.0 g L-1 CH was the most favorable medium composition in enhancing the productions of all antioxidants studied. The pH of MS medium varying from 5.1 to 5.9 did not show any significant effect on the antioxidant activity. Continuous illumination with higher light intensities increased the production of ascorbic acid and α-tocopherol. Carotenoid production was reduced at higher light intensities while light photoperiod has no significant effect on carotenoid content. The above results indicated that the antioxidants produced can be modulated by manipulating the medium composition and physical culture growth conditions in in vitro callus of C. asiatica.

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