Induction of shoots and roots from vegetative tissue culture of Hevea brasiliensis RRIM 2020

C. H., Nor Mayati and A. R., Jamnah (2014) Induction of shoots and roots from vegetative tissue culture of Hevea brasiliensis RRIM 2020. Journal of Tropical Plant Physiology, 6. pp. 1-9.

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Abstract

Adventitious roots and shoot formation were induced from lateral meristem and shoot apical meristem of in vitro grown seedling of Hevea brasiliensis RRIM 2020 clone. Concurrently, shoots and roots were also induced for axillary buds (nodules) from matured bud stumps of this clone. The highest callogenesis at 93% was achieved from lateral meristem culture of RRIM 2020 initiated on Woody Plant media (WPM)-A which was WPM supplemented with 0.1 mg L-¹ benzylaminopurine (BA), 0.5 mg L-¹ 2,4-dinitrophenylhydrazine (2,4-D) and 3% sucrose. The second highest callogenesis at 61% was obtained from lateral meristem culture in WPM-C which was WPM supplemented with 0.5 mg L-¹ BA, 0.5 mg L-¹ 2,4-D, 0.5 mg/L zeatin, 10% coconut water (cw) and 7% sucrose, and this was followed by 49% callogenesis obtained from lateral meristem culture in Murashige and Skoog-Indian modification containing zeatin (MS(ID)Z). Embryogenesis induced in differentiation media, RD1 supplemented with kinetin or zeatin with a combination of plant growth hormones such as benzylaminopurine (BA), indolebutyric acid (IBA), 2,4-D and naphthaleneacetic acid (NAA) has successfully generated rooted embryoids. Rooting of lateral meristem culture was induced from calli initiated on WPM(C) containing 0.5 mg L-¹ zeatin without casein hydrolysate. Meanwhile, the shoot apical meristem culture of RRIM 2020 initiated on developmental media, DM04 and a modified WPM (WPM(B)-M) has successfully enhanced shoot growth to 79% and 36% respectively. Apparently, adventitious shoots regeneration of shoot apical meristem was induced with two different meristem culture media containing thidiazuron (TDZ) (MC(A)-M and modified MC(B)-M). In both cases, while elongation and expansion of roots appeared to be retarded, further shoot development was triggered when the apical meristem was cultured on MS(ID)Z.

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