Isolation of ripening-related genes of Capsicum annuum via a differential display technique

Zamri, Z. and Roslinda, S. and Ismanizan, I (2006) Isolation of ripening-related genes of Capsicum annuum via a differential display technique. Journal of Tropical Plant Physiology, 1 (1). pp. 100-107.

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Abstract

A mRNA differential display was established to allow the isolation and identification of genes induced during chili fruit ripening grow under normal conditioned. Twenty eight PCR-fragments from the differential display were identified using twenty four primer combinations. Only four cDNA fragments were successfully cloned and sequenced. Database searches revealed that CUKMD 1 encodes §-carotene desaturase and CUKMD2 was identified as encoding O-methyltransferases. The fragment CUKMD3 shares 100% homology at amino acid level to catalase isolated from Capsicum annuum. While CUKMD4 showed considerable homology with an amino acid transporter. These studies showed that mRNA differential display is a powerful tool to facilitate the isolation and identification of genes induced during chili fruit ripening.

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