Zamri, Z. and Roslinda, S. and Ismanizan, I (2006) Isolation of ripening-related genes of Capsicum annuum via a differential display technique. Journal of Tropical Plant Physiology, 1 (1). pp. 100-107.
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Abstract
A mRNA differential display was established to allow the isolation and identification of genes induced during chili fruit ripening grow under normal conditioned. Twenty eight PCR-fragments from the differential display were identified using twenty four primer combinations. Only four cDNA fragments were successfully cloned and sequenced. Database searches revealed that CUKMD 1 encodes §-carotene desaturase and CUKMD2 was identified as encoding O-methyltransferases. The fragment CUKMD3 shares 100% homology at amino acid level to catalase isolated from Capsicum annuum. While CUKMD4 showed considerable homology with an amino acid transporter. These studies showed that mRNA differential display is a powerful tool to facilitate the isolation and identification of genes induced during chili fruit ripening.
| Item Type: | Article |
|---|---|
| Creators: | Zamri, Z. and Roslinda, S. and Ismanizan, I |
| Title: | Isolation of ripening-related genes of Capsicum annuum via a differential display technique |
| Date: | December 2006 |
| Location: | The Malaysian Society of Plant Physiology website |
| Publication: | Malaysian Society of Plant Physiology |
| Volume: | 1 |
| Number: | 1 |
| Physical Description: | 8p. |
| Date Deposited: | 02 Nov 2017 01:07 |
| Last Modified: | 02 Nov 2017 01:30 |
| URI: | http://myagric.upm.edu.my/id/eprint/11367 |
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